Before we discuss immunofluorescence, we need to know what fluorescence is. There are certain substances, composed of molecules that will emit light when irradiated by a short wavelength, such as X-rays or ultraviolet (UV) light.
Anthony van Leeuwenhoek is credited with being the first person to use a microscope to view tiny “animacules” in 1674. At approximately the same time, Robert Hooke used a similar microscope to view thin slices of cork. The structures that he observed resembled the tiny cells that monks lived in at the local monastery, so he named the structures “cells”.
The most noticeable tissue processing artefacts are the wrinkles and tears in the tissue sections which are evident even at low power
Standard tissue processing may be carried out on any number of open and closed tissue processors, although closed processors are preferred due to safety concerns, both for the tissues and laboratory personnel.
The first four blogs of the troubleshooting series focused on being proactive with regard to the prevention of sub-optimal events in the histology laboratory
Some specimens may be very tiny; on the order of less than 0.1 cm. Some preparation methods employ the use of mesh cassettes, “tea bag” biopsy pouches, sponges, wrapping paper, etc. to contain the specimen and prevent it from escaping the tissue processing cassette.
No matter what type of histology laboratory you work in – hospital, research, reference, teaching facility – there will be times when you receive specimens that you do not normally receive.
In the previous blog we looked at one way to minimize troubleshooting by being proactive and looking ahead to possible situations and procedures that exist in your laboratory that may cause sub-optimal events. This blog will continue with that same mind set.
A definition of troubleshooting in histology is: the identification of the cause of a sub-optimal event which occurs in the laboratory and the successful implementation of the corrective action of the event.
If Prince Hamlet from William Shakespeare’s play Hamlet was living today as a laboratory scientist, he might be pondering, “To duct, or not to duct, that is the question”. This would be very fitting because an informed decision is needed when it comes time for you to select a ducted or ductless chemical fume hood for your facility.