Why even bother to learn about troubleshooting? Wouldn’t it be more interesting to listen to one of the presentations on molecular technology? Possibly. But what would you do if your …
Congratulations on purchasing a new autostainer! There are three parts to the start-up process: Instrument Verification, Stain Protocol Optimization, and Validation of the Staining Protocols. Instrument Verification – see CAP …
The DNA of a nucleus directs the actions of the cell by instructing it to manufacture specific proteins. This “instruction” is sent from the nucleus into the cytoplasm by “messenger …
Immunohistochemistry Educational Series In previous installments of the IHC Educational Series, we discussed the specifics of the basic procedures found in the IHC laboratory: immunofluorescence, immunohistochemistry and in situ hybridization. …
In the previous segments of the IHC Educational Series, immunofluorescence and immunoperoxidase methods were discussed. In both of these methods, antibodies are used to bind to and localize protein antigens …
In the previous units of this series, the immunohistochemical (IHC) methods of immunofluorescence and immunoperoxidase were described and discussed. These methods use antibodies to localize cellular proteins in tissue sections, …
In the previous segment, immunofluorescence methods were discussed. These methods use antibodies labelled with fluorescein to directly localize cellular proteins in tissue sections, which can then be visualized in a …
Before we discuss immunofluorescence, we need to know what fluorescence is. There are certain substances, composed of molecules that will emit light when irradiated by a short wavelength, such as …
Anthony van Leeuwenhoek is credited with being the first person to use a microscope to view tiny “animacules” in 1674. At approximately the same time, Robert Hooke used a similar …
This blog is a followup to the previous article “The H&E Stain: Far From Routine”. In that article, the basics of the routine hematoxylin and eosin (H&E) stain were discussed. …
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