Posts filed under: Stains

This blog is a followup to the previous article “The H&E Stain: Far From Routine”.  In that article, the basics of the routine hematoxylin and eosin (H&E) stain were discussed.  Now we shall discuss how to trouble shoot the routine...
[Editor’s note:  Segments of following blog are taken directly from an original article “The H&E Stain: Far from Routine” published by the author in Advance for Medical Professionals in April 2002.]   What exactly is a routine “H&E”?  And what...
In the histology world, the mere mention of a “silver stain” may be the cause of panic and uncertainty with regard to the performance of the stain, and the quality of the final resulting microscope slide.  All other special stains,...
The staining of microorganisms in histology can be challenging. Filamentous fungi and associated conidia are more easily demonstrated as they are visible under light microscopy when stained with periodic acid Schiff’s (PAS) as in Figure 1. The diameter of fungi...
Fungi include molds, yeasts and higher fungi. All fungi are eukaryotic and have sterols but not peptidoglycan in their cell membrane. Their cell walls are composed of cellulose; the same building blocks that plants use. Fungi may produce large, reproductive...
When staining sections for the presence of carbohydrates, the two main classes under investigation are glycogen and mucins.  Mucins include substances referred to as mucopolysaccharides, mucosubstances, glycoproteins and glycoconjugates. Mucins provide an environment that is conducive to molecular diffusion of...